Boosting the free fatty acid synthesis of Escherichia coli by expression of a cytosolic Acinetobacter baylyi thioesterase

Yan-Ning Zheng Lingling Li Hui Liu Guang Zhao

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   https://www.researchgate.net/publication/232234433

.......All restriction enzymes and T4 DNA ligatase were purchased from Fermentas (Vilnius, Lithuania). The PrimeSTAR HS DNA polymerase was supplied by Takara Biotechnology (Dalian, China). Oligonucleotides were ordered from Generay Biotechnology (Shanghai, China). The arachidic acid was ordered from Alfa Aesar (Ward Hill, MA)......