Oncolytic and immunologic cancer therapy with GM-CSF-armed vaccinia virus of Tian Tan strain Guang9

·   Lili Deng ,  

·   Jun Fan ,  

·   Mingming Guo ,  

·   Biao Huang

·  

Cancer Letters   Volume 372, Issue 2 , 28 March 2016, Pages 251–257

http://dx.doi.org/10.1016/j.canlet.2016.01.025

·  ……Viral DNA was extracted by Generay kit (Shanghai Generay Biotech Co., Ltd). A standard PCR was performed using primers external to the site of recombination
(P1: 5′-ATGAACGGCGGACATATTCA-3′; P2: 5′-TATGAGTCGATGTAACACTTTC-3′) and within the GM-CSF gene……