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Sedimentary DNA reveals over 150 years of ecosystem change by human activities in Lake Chao, China

Sedimentary DNA reveals over 150 years of ecosystem change by human activities in Lake Chao, China


Feilong Lia , Xiaowei Zhanga,, Yuwei Xieb , Jizhong Wangc,d a State Key Laboratory of Pollution Control & Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, PR China b Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B3, Canada c Guangzhou GRG Metrology & Test (Hefei) CO., LID, Hefei 230088, PR China d School of Environment and Energy Engineering, Anhui Jianzhu University, Hefei 230601, PR China



Universal eukaryotic primers (1380F: TCCCTGCCHTTTGTACACAC; 1510R: CCTTCYGCAGGTTCACCTAC) with attached unique 12-nt nucleotides (also known as tags) were used to amplify the 130 bp fragment of the V9 region of 18S rRNA genes (Amaral-Zettler et al., 2009). Universal bacterial primers (341F: ACCTACGGGRSGCWGCAG; 518R: GGTDTTACCGCGGCKGCTG) with attached unique tags were used to amplify the 180 bp fragment of the V3 region of 16 s rRNA genes (Klindworth et al., 2013; Muyzer et al., 1993). Primers were synthesized by Shanghai Generay Biotech Co., Ltd. Each sample was amplifified in triplicate PCR replicates and was subsequently pooled for the next procedure



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