As an important research tool, RNA is widely used in gene function analysis, development of novel therapeutic strategies and other research applications. We provide high-quality customized RNA synthesis services to meet the different needs of our customers, including general RNA synthesis, RNA modification and labeling, chimeric RNA, 2'-OMe-RNA and other antisense RNAs synthesis, etc. To ensure the high quality of RNA synthesis, we strictly carry out QC testing standards through MS, HPLC, and other methods. In order to ensure the high quality of RNA, the synthesized RNA is strictly implemented QC testing standards, through MS, HPLC and other testing methods to ensure that the final products delivered to customers are high quality RNAs.

siRNA

siRNA is a 19~25nt double-stranded RNA small molecule, which is a conventional tool used for gene silencing with low cost and high reproducibility, and has been widely used in biomedical research and clinical drug development. GENERAY Biotechnology can provide customers with high-quality, highly reproducible and customized siRNA services for preclinical studies in cells and animals. All of our siRNA products are purified by HPLC to completely remove the unpaired single strand, and customers only need to dilute them with the buffer in the box for subsequent experiments.

1.1  Single siRNA customization

Conventional siRNA (15-25bp double-stranded RNA, suitable for general cellular experiments)

Chemically modified siRNA (applicable to animal experiments, siRNA stability and silencing efficiency are enhanced, and the duration of gene silencing is extended)

1.2  siRNA package

Conventional siRNA package (3 for 1 package, ensuring at least one pair of siRNA knockdown efficiency of 70% or more, suitable for general cell experiments)

Chemically modified siRNA package (suitable for animal experiments, siRNA stability and silencing efficiency are enhanced, and the duration of gene silencing is extended)

miRNA

Generay Biotechnology miRNA products mainly include miRNA Mimics (double-stranded annealing products), miRNA Mimic negative control, miRNA inhibito (r RNA single-stranded), and miRNA inhibitor negative control products. Our miRNA products are manufactured under the ISO9001:2015 quality standard and have been optimized and rigorously tested, with highly stable quality.

2.1  Common miRNA synthesis price list (15-25bp) double-stranded/single-stranded

2.2  Modified miRNA synthesis price list (15-25bp) double-stranded/single-stranded

sgRNA and single-stranded RNA

CRISPR/Cas9 has been widely used in Knock out (KO), Knock in (KI), Repression / Activation (CRISPRi / CRISPRa) and other experiments. As a leading company in gene synthesis, Shanghai GENERAY Biotechnology provides sgRNA design and synthesis, CRISPR/Cas9 plasmid construction (three guarantees and one package). We will provide the following services to assist the development of gene editing drugs and related R&D projects.

gRNA and CRISPR/Cas9 plasmids

Conventional CRISPR plasmids: pSpCas9(BB)-2A-GFP (abbreviation: PX458, GFP tag), pSpCas9(BB)-2A-Puro (abbreviation: PX459, Puro tag) V2.0 Conventional lentiviral plasmid: lentiCRISPR v2 (Puro tag)

Large-scale RNA synthesis

Large-scale RNA oligonucleotide synthesis ranges from 50 mg to several grams. To ensure the highest quality RNA, all large-scale RNA oligonucleotide synthesis is processed and purified in an RNase-free environment. All large-scale RNA oligonucleotides were analyzed by HPLC, PAGE, and ESI-MS mass spectrometry.

Service Features

✓ 50 mg to several grams. Length 5-50 nt.

✓ Phosphorothioate (PS), phosphodiester (PO) or PO/PS backbone.

✓ Chimeric oligonucleotides mixed with 2'-OMe, 2'-F-RNA, MOE, LNA or DNA. various oligonucleotide modifications: dyes, spacer groups, junctions.

✓ Multiple purification options available, desalting or HPLC purification, etc.

✓ Multiple chemistry needs such as sterilization/filtration, endotoxin testing, Na+ salt exchange, etc. can be selected. Quality control by HPLC, PAGE, ESI mass spectrometry.

✓ Preferential pricing is available through supply agreements.

Oligos and other couplers

Generay Biotechnology can provide PEG, Peptide, chelating agent (NOTA, DOTA, MAG, DTPA), etc., and other small molecules coupling service with Oligo.

Quality Assurance

✓ Complete order service system

✓ All RNA oligonucleotides are produced under strict SOP quality control process

✓ High resolution Waters chromatography with ESI-MS to ensure purity

Delivery results

✓ Synthetic RNA lyophilized powder

✓ COA documentation

✓ Assay report (MS & HPLC)

Transfection Reagent

Intracellular delivery of DNA and RNA (cell transfection) is a fundamental technology in the life sciences. Successful transfection is crucial for biological research, including gene overexpression, gene silencing (i.e. RNAi), genome editing and other biomedical research. Jereh Bio offers a wide range of transfection reagents for DNA, RNA and in vivo injection with high efficiency and low toxicity.

1. GyTrans™-RNA

GyTrans™-RNA can be used to transfect siRNA, miRNA, circRNA, sgRNA and other products. It is serum-independent and does not require media changes. It is suitable for primary cells, conventional cell lines and difficult-to-transfect cell lines.

2. GyTrans™-DNA

GyTrans™-DNA can be used for transfection of overexpression plasmids, shRNA plasmids, CRISPR plasmids, viral plasmids and so on. It is suitable for primary cells, conventional cell lines and difficult-to-transfect cell lines.

3. GyTrans™-RNA&DNA

GyTrans™-RNA&DNA can be used to transfect plasmid DNA, siRNA, miRNA, circRNA, sgRNA and other products. It is suitable for primary cells, conventional cell lines and difficult-to-transfect cell lines.

4. GyTrans™-Suspension

GyTrans™-Suspension can be used for routine transfection of suspension cells in the laboratory, industrialized large-scale protein production. It is serum-independent and does not require media changes. It is suitable for 293F, CHO-S and other suspension cell lines.

5. GyTrans™-vivo

GyTrans™-vivo is a high performance in vivo transfection reagent. Compared with other transfection reagents, GyTrans™-vivo has the advantages of excellent transfection effect, low in vivo toxicity, good stability, high reproducibility, etc. GyTrans™-vivo is suitable for in vivo DNA/RNA transfection in animals such as rats, mice, rabbits, swine, and monkeys. GyTrans™-vivo can be used for tumor-bearing mice, animal gene editing, and animal modeling. GyTrans™-vivo is suitable for tail vein injection and local injection.

Transfection Reagent Quotation

Contact Information

●   By Email: rna@generay.com.cn

●   By Phone: 86-21-67840801/0802