Standard Custom Oligos


Comparison Table Of Synthetic Specifications And Final Yield(15-60nt)

 

Purification  Method

Synscale

Final  Yield

OPC

50nmol

2OD

100nmol

5OD

200nmol

10OD

PAGE

50nmol

1OD

100nmol

2OD

200nmol

3OD

HPLC

50nmol

1OD

100nmol

2OD

200nmol

5OD

 

 

Generay Price Of Different Purification Options And Primer Length

 

1. Introduction and price list of OPC purification primers

Purification with reverse-phase silica gel column which can effectively remove salts. The purity can meet the needs of most molecular biology experiments.

 

Advantages

●   Fast

●   High throughput

●   Cost-effective

 

Applications

PCR amplification, gene synthesis, DNA sequencing, NGS capture probes, NGS Multiplex PCR oligos.


Primer length

purification method

yield

Price($)/nt

Price($)/purified

15-60nt

OPC

2OD

0.16 /nt

/

15-60nt

OPC

5OD

0.20 /nt

/

15-60nt

OPC

10OD

0.34 /nt

/

15-60nt

OPC

>10OD

Inquiry

/


2. Introduction and price list of PAGE purification primers

Using denaturing polyacrylamide gel electrophoresis, oligo of different molecular weights migrate at different speeds during electrophoresis, allowing for separation. Finally, the target DNA is recovered from the gel.

 

Advantages

●   Purity can be > 90%

●   Effective for purification of long-stranded oligo

 

Applications

Mutation libraries, protein binding gel migration electrophoresis analysis, modified oligos such as phosphate and biotin.


Primer Length

Purification Method

Yield

Price($)/nt

Price($)/purified Markup

15-60nt

PAGE

2OD

0.21/nt

5.00/ purified

15-60nt

PAGE

5OD

0.35/nt

7.00/ purified

15-60nt

PAGE

10OD

0.45/nt

9.00/ purified

15-60nt

PAGE

>10OD

Inquiry

Inquiry

* Markup prices are PAGE purification fees only


3.Introduction and price list of HPLC purification primers

The purification of oligo with HPLC can achieve high purity and is mainly used for short chain and modified oligo.

 

Advantages

●   Purity can reach > 90%

●   Remove N-1 short fragment effectively

●   Purification for modified oligo with hydrophobic groups

 

Applications

Point mutation, subcloning, diagnostic PCR protein binding gel migration electrophoresis analysis, modified primers with hydrophobic groups.

 

Primer Length

Purification Method

Yield

Price($)/nt

Price($)/purified Markup

15-60nt

HPLC

2OD

0.21/nt

9.00 /purified

15-60nt

HPLC

5OD

0.35 /nt

12.00/ purified

15-60nt

HPLC

10OD

0.45/nt

13.50/ purified

15-60nt

HPLC

>10OD

Inquiry

Inquiry

61-90nt

HPLC

2OD

0.40nt

11.00/purified

61-90nt

HPLC

5OD

0.60/nt

13.00/purified

61-90nt

HPLC

10OD

0.90/nt

16.00/purified

61-90nt

HPLC

>10OD

Inquiry

Inquiry

90-130nt

HPLC

1OD

0.60/nt

13.00/purified

90-130nt

HPLC

2OD

0.60/nt

13.00/purified

90-130nt

HPLC

5OD

0.90/nt

16.00/purified

90-130nt

HPLC

>5OD

Inquiry

Inquiry

* Markup prices are HPLC purification fees only



Contact Information

 

●   By Email: dna@generay.com.cn

●   By Phone: 86-21-67840801/0802



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