Modified Oligos
The addition of modification groups with various functions during oligo synthesis can support molecular biology studies for a variety of different purpose. There are five types according to the position of primer modification：5’-end modifications；3’-end modifications；2’-end modifications；Intermediate modifications；Nucleotide variant modifications.
DNA Modification Types Information
Here is a list of DNA modification and modification position that Generay can provide，Other modifications can be evaluated based on the availability of raw materials.
Competitive Advantages
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Modification name | code | 5' -End Modifications | 3' -End Modifications | Intermediate Modifications |
Fluorophore | ||||
AMCA | AMCA | √ | √ | √ |
Pacific Blue | Pacific Blue | √ | √ | √dT |
Atto 425 | Atto 425 | √ | √ | √dT |
BODIPY FL | BODIPY FL | √ | √ | √dT |
FAM | 6-FAM | √ | √ | √dT |
Alexa Fluor 488 | Alexa Fluor 488 | √ | √ | √dT |
TET | TET | √ | √ | √dT |
JOE | JOE | √ | √ | √dT |
Yakima Yellow | Yakima Yellow | √ | √ | √ |
VIC | VIC | √ | √ | √ |
HEX | HEX | √ | √ | √dT |
Quasar 570 | Quasar 570 | √ | √ | √dT |
Cy3 | Cy3 | √ | √ | √dT √ boned |
NED | NED | √ | √ | √ |
TAMRA | TAMRA | √ | √ | √dT |
ROX | ROX | √ | √ | √dT |
AquaPhluor 593 | AquaPhluor 593 | √ | ||
Texas red | Texas red | √ | √ | √dT |
Atto 590 | Atto 590 | √ | √ | √ |
Cy5 | Cy5 | √ | √ | √dT √ boned |
Quasar 670 | Quasar 670 | √ | √ | √ |
Cy5.5 | Cy5.5 | √ | √ | √dT |
Cy7 | Cy7 | √ | √ | √dT |
Quenching | ||||
Dabcyl | Dabcyl | √ | √ | √dT |
Eclipse | Eclipse | √ | ||
MGB | MGB | √ | √ | |
BHQ1 | BHQ1 | √ | √ | √dT |
BHQ2 | BHQ2 | √ | √ | √dT |
BHQ3 | BHQ3 | √ | √ | √ |
Linker | ||||
Amino | NH2 | √C6 √C12 | √C6 √C7 | √dT |
Int Uni-Link Amino | √ | √dT | ||
Carboxy | COOH | √C10 | ||
Aldehyde | CHO | √C2 | ||
Thiol | SH | √C6 | √C3 √C6 | √ |
HS-SH | √C6 | √C3 √C6 | √boned | |
Dithiol | √ | √ | √dT √ boned | |
Triple SH | √ | |||
Maleimide | Maleimide | √ | √ | √ |
Acrydite | Acrydite | √ | √ | √dT |
Alkyne | C≡CH | √ | √ | √dT |
Azide | N3 | √ | √ | √dT |
DBCO | DBCO | √ | √ | √dT |
Biotin | Biotin | √ | √ | √dT |
Biotin TEG | √ | √ | ||
Desthio Biotin | √ | |||
Desthio Biotin TEG | √ | √ | √dT | |
Dual Biotin | √ | |||
Triple Biotin | √ | |||
Digoxin | Dig | √ | √ | √dT |
Nucleoside Analogues | ||||
2-Aminopurine | 2-Aminopurine | √ | √ | √boned |
5-Bromo dU | 5-Br dU | √ | √ | √boned |
DeoxyUridine | dU | √ | √ | √boned |
Inverted dT | Inverted dT | √ | ||
Inverted dG | Inverted dG | √ | ||
Dideoxycytidine | ddC | √ | ||
5-Methyl dC | 5-Me dC | √ | √ | √boned |
5-Hydroxymethyl dC | 5-Hm dC | √ | √ | √boned |
N6-Methyl dA | N6-Me-dA | √ | √ | √boned |
5-Aza-2’-dC | 5-Aza-2’-dC | √ | √ | √boned |
deoxyInosine | dI | √ | √ | √boned |
5-Nitroindole | 5-Nitroindole | √ | √ | √boned |
LNA | XNA | √ | √ | √boned |
2`-O-Methyl Base | 2`-O-Methyl Base | √ | √ | √boned |
RNA | rA/rG/rC/rU | √ | √ | √boned |
2' Fluoro bases | 2-F RNA | √ | √ | √boned |
Pyrrolo-dC | Pyrrolo-dC | √ | √ | √boned |
8-Oxo deoxyguanosine | 8-oxo-dG | √ | √ | √boned |
Phosphorylation | P | √ | √ | |
phosphorothioate | * | √boned | ||
Others | ||||
Pyrene | Pyrene | √ | ||
Symmetric | Symmetric | √ | √ | |
Puromycin | Puromycin | √ | ||
Cholesterol | Cholesterol | √ | √ | |
Azobenzene | Azobenzene | √ | √ | |
Methylene Blue | Methylene Blue | √ | √ | √dT |
Ferrocene | Ferrocene | √ | √ | √dT |
Ru | Ru | √ | √ | √dT |
Introduction To Modified/Labled Primers With Quotation
（1）Modified by thiocyanate, modified by Di, Du, 2' -O-ME-base and 5-methyl DC
Modified Category | purification method | specification | price ($) |
Thio modification * | HPLC | 2 | 1.00 |
5 | 1.20 | ||
10 | 1.50 | ||
50 | 5.00 | ||
（DeoxyInosine） dI | HPLC | 2 | 13.00 |
5 | 15.00 | ||
10 | 19.00 | ||
（DeoxyUridine） dU | HPLC | 2 | 14.00 |
5 | 16.00 | ||
10 | 24.00 | ||
5-Methyl dC | HPLC | 2 | 45.00 |
5 | 55.00 | ||
10 | 70.00 | ||
2'-O-Me- C | HPLC | 2 | 12.00 |
5 | 15.00 | ||
10 | 23.00 | ||
2'-O-Me- G | HPLC | 2 | 12.00 |
5 | 15.00 | ||
10 | 23.00 | ||
2'-O-Me- U | HPLC | 2 | 12.00 |
5 | 15.00 | ||
10 | 23.00 | ||
2'-O-Me- A | HPLC | 2 | 12.00 |
5 | 15.00 | ||
10 | 23.00 | ||
5-Carboxy-dC | HPLC | 2 | Inquiry |
5-Hydroxymethyl-dC | HPLC | 2 | Inquiry |
5-Formyl-dC | HPLC | 2 | Inquiry |
N6-Me-dA | HPLC | 2 | Inquiry |
2’-F-base | HPLC | 2 | 35.71 |
5 | 50.00 | ||
10 | 71.43 | ||
Some of the above modifications are modified, and the modification bits are billed according to the price in the table, and the other bits are charged by ordinary DNA bases. For other special base modifications, please request a quote. | |||
（2）Phosphorylation, amino, sulfhydryl, and various spacers modifications
Modification Category | Modification Method | Specification (OD)/Price ($) | ||
2 OD | 5 OD | 10 OD | ||
Phosphorylation | 5'-Phosphorylation | 24.00 | 26.00 | 32.00 |
3'-Phosphorylation | 24.00 | 26.00 | 32.00 | |
Amino | 5'-Amino | 23.00 | 29.00 | 43.00 |
3'-Amino | 23.00 | 29.00 | 43.00 | |
5'-NH2 C12 | 72.00 | 86.00 | 114.00 | |
Spacers | Spacer C3 | 43.00 | 50.00 | 86.00 |
Spacer 9 | 60.00 | 71.00 | 86.00 | |
Spacer 18 | 60.00 | 71.00 | 86.00 | |
Thiolation | 5'-SH C6 | 58.00 | 71.00 | 114.00 |
3'-SH C6(3’-SH C3) | 58.00 | 71.00 | 114.00 | |
3'-diThiol* | 114.00 | 143.00 | 215.00 | |
Biotin | 5'- Biotin | 30.00 | 38.00 | 54.00 |
5'-Biotin TEG | 58.00 | 71.00 | 114.00 | |
3'- Biotin | 32.00 | 40.00 | 58.00 | |
3'- Biotin TEG | 43.00 | 65.00 | 86.00 | |
Digoxin | 5'- Digoxin | 72.00 | 100.00 | 143.00 |
3'- Digoxin | 86.00 | 114.00 | 171.00 | |
Inverted dT | 3'-Inverted dT | 43.00 | 50.00 | 71.00 |
ddC | 3'-ddC | 72.00 | 86.00 | 129.00 |
（3）Fluorogenic modifications
Modification Category | Modification Method | Specification (OD)/Price ($) | ||
2 OD | 5 OD | 10 OD | ||
FAM | 5'- FAM | 35.00 | 43.00 | 65.00 |
3'- FAM | 35.00 | 43.00 | 65.00 | |
FITC | 5'- FITC | 35.00 | 43.00 | 65.00 |
3'- FITC | 35.00 | 43.00 | 65.00 | |
5'- HEX | 50.00 | 54.00 | 75.00 | |
3'- HEX | 50.00 | 54.00 | 75.00 | |
SIMA | 5'-SIMA | 85.00 | 155.00 | 280.00 |
JOE | 5'- JOE | 55.00 | 70.00 | 100.00 |
3'- JOE | 65.00 | 80.00 | 120.00 | |
ROX | 5'- ROX | 55.00 | 70.00 | 100.00 |
3'- ROX | 65.00 | 80.00 | 120.00 | |
Rhodamine Green | 5'- Rhodamine Green | 85.00 | 157.00 | 285.00 |
3'- Rhodamine Green | 85.00 | 157.00 | 285.00 | |
Rhodamine Red | 5'- Rhodamine Red | 85.00 | 157.00 | 285.00 |
3'- Rhodamine Red | 85.00 | 157.00 | 285.00 | |
TAMRA | 5'- TAMRA | 50.00 | 60.00 | 90.00 |
3'- TAMRA | 55.00 | 65.00 | 100.00 | |
TET | 5'- TET | 50.00 | 54.00 | 75.00 |
3'- TET | 50.00 | 54.00 | 75.00 | |
Texas Red | 5'- Texas Red | 71.00 | 86.00 | 129.00 |
3'- Texas Red | 71.00 | 86.00 | 129.00 | |
Cy3 | 3' -Cy3 | 75.00 | 95.00 | 130.00 |
5' -Cy3 | 68.00 | 70.00 | 100.00 | |
Cy5 | 3' -Cy5 | 75.00 | 95.00 | 115.00 |
5' -Cy5 | 70.00 | 85.00 | 110.00 | |
CY5.5 | 3' -CY5.5 | 171.00 | 257.00 | 429.00 |
5' -CY5.5 | 143.00 | 214.00 | 357.00 | |
AMCA | 3' - AMCA | 71.00 | 86.00 | 129.00 |
5' - AMCA | 71.00 | 86.00 | 129.00 | |
Methylene Blue | 3' -Methylene Blue | 129.00 | 243.00 | 400.00 |
5' -Methylene Blue | 114.00 | 214.00 | 357.00 | |
BHQ -1 | 3’BHQ -1 | 55.00 | 70.00 | 100.00 |
5’BHQ -1 | 80.00 | 95.00 | 125.00 | |
BHQ -2 | 3’BHQ -2 | 55.00 | 70.00 | 100.00 |
5’BHQ -2 | 71.00 | 86.00 | 114.00 | |
3’Eclipse | 3’Eclipse | 71.00 | 86.00 | 114.00 |
Dabsyl | 3' Dabsyl | 71.00 | 86.00 | 114.00 |
Dabcyl | 3' -Dabcyl | 71.00 | 86.00 | 114.00 |
5' -Dabcyl | 71.00 | 86.00 | 114.00 | |
Fluorescein | Fluorescein | 157.00 | 200.00 | 257.00 |
Ferrocene | 3' -Ferrocene | 71.00 | 86.00 | 129.00 |
5' -Ferrocene | 57.00 | 71.00 | 114.00 | |
Cholesteryl | 3' -Cholesteryl | 57.00 | 71.00 | 114.00 |
5' -Cholesteryl | 57.00 | 71.00 | 114.00 | |
ATTO425 | 3' - ATTO425 | 171.00 | 200.00 | 286.00 |
5' - ATTO425 | 171.00 | 200.00 | 286.00 | |
ATTO550 | 3' - ATTO550 | 171.00 | 200.00 | 286.00 |
5' - ATTO550 | 171.00 | 200.00 | 286.00 | |
ATTO565 | 3' - ATTO565 | 114.00 | 129.00 | 171.00 |
5' - ATTO565 | 114.00 | 129.00 | 171.00 | |
ATTO633 | 3' - ATTO633 | 186.00 | 214.00 | 300.00 |
5' - ATTO633 | 186.00 | 214.00 | 300.00 | |
Yakima Yellow | 3' -Yakima Yellow | 114.00 | 129.00 | 171.00 |
5' -Yakima Yellow | 114.00 | 129.00 | 171.00 | |
iFluor™ 488 | 3' - iF488 | 243.00 | 314.00 | 471.00 |
5' - iF488 | 243.00 | 314.00 | 471.00 | |
iFluor™ 532 | 3' - iF532 | 243.00 | 314.00 | 471.00 |
5' - iF532 | 243.00 | 314.00 | 471.00 | |
iFluor™ 555 | 3' - iF555 | 243.00 | 314.00 | 471.00 |
5' - iF555 | 243.00 | 314.00 | 471.00 | |
iFluor™ 648 | 3' - iF648 | 243.00 | 314.00 | 471.00 |
5' - iF648 | 243.00 | 314.00 | 471.00 | |
iFluor™ 750 | 3' - iF750 | 243.00 | 314.00 | 471.00 |
5' - iF750 | 243.00 | 314.00 | 471.00 | |
（4）MGB probes
5'- fluorophore | 3'- quencher | Specification (OD)/Price ($) | ||
2 OD | 5 OD | 10 OD | ||
5’-FAM | 3'-MGB | 210.00 | 230.00 | 300.00 |
5’-HEX | 145.00 | 185.00 | 200.00 | |
5’-VIC | 190.00 | 240.00 | 400.00 | |
5’-NED | 3'-MGB | 180.00 | 220.00 | 240.00 |
5’-ROX | 140.00 | 180.00 | 195.00 | |
5’-CY5 | 180.00 | 220.00 | 240.00 | |
（5）Dual-labeled Fluorogenic Probes
5'- fluorophore | 3'- quencher | Specification (OD)/Price ($) | ||
2 OD | 5 OD | 10 OD | ||
5'- FAM | 3'- TAMRA | 85.00 | 110.00 | 165.00 |
3'- Dabcyl | 105.00 | 130.00 | 180.00 | |
3'- BHQ1 | 85.00 | 110.00 | 165.00 | |
3'-Eclipse | 142.00 | 165.00 | 250.00 | |
5'- HEX | 3' TAMRA | 110.00 | 135.00 | 185.00 |
3'- Dabcyl | 105.00 | 130.00 | 180.00 | |
3'- BHQ1 | 85.00 | 110.00 | 165.00 | |
3'- BHQ2 | 85.00 | 110.00 | 165.00 | |
3'-Eclipse | 142.00 | 165.00 | 250.00 | |
5'-TET | 3'- Dabcyl | 105.00 | 130.00 | 180.00 |
3'- TAMRA | 110.00 | 135.00 | 185.00 | |
3'- BHQ1 | 85.00 | 110.00 | 165.00 | |
3'- BHQ2 | 85.00 | 110.00 | 165.00 | |
3'-Eclipse | 142.00 | 165.00 | 250.00 | |
5'- JOE | 3'- TAMRA | 110.00 | 135.00 | 185.00 |
3'- Dabcyl | 105.00 | 130.00 | 180.00 | |
3'- BHQ1 | 105.00 | 130.00 | 180.00 | |
5'-ROX | 3'- BHQ2 | 110.00 | 145.00 | 190.00 |
3'-Eclipse | 105.00 | 130.00 | 180.00 | |
5'- Cy3 | 3'- Dabcyl | 115.00 | 135.00 | 190.00 |
3'-Eclipse | 110.00 | 130.00 | 180.00 | |
3'- BHQ2 | 135.00 | 200.00 | 250.00 | |
5'- Cy5 | 3'- Dabcyl | 115.00 | 135.00 | 190.00 |
3'-Eclipse | 110.00 | 130.00 | 180.00 | |
3'- BHQ2 | 135.00 | 200.00 | 250.00 | |
3'- BHQ3 | 150.00 | 250.00 | 320.00 | |
5'- Cy5.5 | 3'- BHQ2 | 230.00 | 300.00 | 430.00 |
3'- BHQ3 | 275.00 | 355.00 | 480.00 | |
5'-Quasar 670 | 3'- BHQ2 | 165.00 | 190.00 | 275.00 |
3'- BHQ3 | 205.00 | 245.00 | 358.00 | |
5'- TAMRA | 3'- BHQ2 | 128.00 | 170.00 | 257.00 |
3'-Eclipse | 257.00 | 170.00 | 257.00 | |
5'-Texas Red | 3'- BHQ2 | 172.00 | 200.00 | 285.00 |
（6）Intermediate modifications
Modification Category | Specification (OD)/Price ($) | ||
2 OD | 5 OD | 10 OD | |
RNA base | 12.50 | 15.50 | 25.00 |
2-Aminopurine | 62.00 | 77.00 | 124.00 |
5-Bromo dU | 62.00 | 77.00 | 124.00 |
8-OXO-dG | 124.00 | 154.00 | 231.00 |
C3 Spacer | 46.50 | 54.00 | 92.50 |
C6 Spacer | 46.50 | 54.00 | 77.00 |
C12 Spacer | 62.00 | 77.00 | 124.00 |
Spacer 9 | 65.00 | 77.00 | 92.00 |
Spacer 18 | 65.00 | 77.00 | 92.00 |
dSpacer | 62.00 | 77.00 | 124.00 |
1-Me-dA | 124.00 | 154.00 | 231.00 |
PC-Linker | 124.00 | 154.00 | 231.00 |
Pyrrolo-dC | 124.00 | 154.00 | 231.00 |
Inverted dA(G、C、T) | 23.00 | 31.00 | 46.00 |
5-Aza-2`-deoxycytidine | 124.00 | 154.00 | 231.00 |
int 6-FAM-dT | 108.00 | 131.00 | 200.00 |
int HEX dT | 124.00 | 154.00 | 246.00 |
int ROX-dT | 138.00 | 169.00 | 262.00 |
int TAMRA-dT | 124.00 | 154.00 | 246.00 |
int TET-dT | 124.00 | 154.00 | 246.00 |
int Texas Red dT | 138.00 | 169.00 | 262.00 |
int Cy5 dT | 185.00 | 231.00 | 354.00 |
int Cy3(backbone) | 124.00 | 154.00 | 231.00 |
int Cy3 dT | 185.00 | 231.00 | 354.00 |
int BHQ1 dT | 138.00 | 169.00 | 246.00 |
int BHQ2 dT | 138.00 | 169.00 | 246.00 |
int NH2 C6 dT | 62.00 | 77.00 | 124.00 |
int Biotin dT | 92.00 | 115.00 | 177.00 |
int DabcyldT | 138.00 | 169.00 | 246.00 |
int Dig dT | 138.00 | 185.00 | 277.00 |
int Dithiol | 124.00 | 154.00 | 231.00 |
int HS-SH C6 | 62.00 | 77.00 | 124.00 |
int Methylene Blue dT | 185.00 | 308.00 | 508.00 |
int AzidedT | 124.00 | 154.00 | 246.00 |
int CHCH dT | 138.00 | 169.00 | 246.00 |
int DBCO dT | 138.00 | 169.00 | 246.00 |
（7）LNA Probes
Modify the group | Specification (OD)/Price ($) | ||
2 OD | 5 OD | 10 OD | |
LNA-dA | 50.00 | 64.00 | 100.00 |
LNA-dG | 50.00 | 64.00 | 100.00 |
LNA-dC | 50.00 | 64.00 | 100.00 |
LNA-dT | 50.00 | 64.00 | 100.00 |
（8）Connecting probe
Modify the group | Specification (OD)/Price ($) | ||
2 OD | 5 OD | 10 OD | |
triple SH | Inquiry | Inquiry | Inquiry |
Maleimide | 114.00 | 143.00 | 171.00 |
Azide | 57.00 | 71.00 | 114.00 |
CHCH | 114.00 | 143.00 | 171.00 |
DBCO | 114.00 | 143.00 | 171.00 |
COOH | 57.00 | 71.00 | 114.00 |
Acrydite | 114.00 | 143.00 | 214.00 |
Hydrazine | Inquiry | Inquiry | Inquiry |
CHO | 57.00 | 71.00 | 114.00 |
Contact Information
● By Email: dna@generay.com.cn
● By Phone: 86-21-67840801/0802
