EndoFree Plasmid Miniprep Kit (Spin Column)
Product Composition
Components | GK2104 25 Preps | GK2104 100 Preps |
Genclean Column GP3 | 25 | 100 |
Collection Tube | 25 | 100 |
Buffer CBS | 10 mL | 25 mL |
Solution Ⅰ | 15 mL | 60 mL |
Solution Ⅱ | 15 mL | 60 mL |
Solution Ⅳ | 12 mL | 50 mL |
Solution GETR | 3 mL | 12 mL |
Solution ePB | 13 mL | 50 mL |
Solution eW1 | 15 mL | 60 mL |
Wash Solution | 6 mL | 24 mL |
Enhanced Elution Buffer | 10 mL | 25 mL |
RNase A (10 mg/mL) | 250 μL | 1 mL |
Protocol | 1 | 1 |
Storage
·RNase A: Store at -20 ℃. Other components are stable for 18 months at room temperature (15-25 ℃). For storage at elevated temperatures or extended shelf life, store at 2-8 ℃
Features
·Flexible and easy to operate.
·Yields 100 μg of high-concentration, endotoxin-free plasmid DNA. Recommended culture volumes: 6-10 mL LB medium or 3-6 mL 2YT medium.