EndoFree Plasmid Midiprep Kit (Spin Column)
EndoFree Plasmid Midiprep Kit (Spin Column)
Product Composition
Components | GK2008 10 Preps | GK2008 50 Preps |
Genclean Column GP4 | 10 | 50 |
5-mL Collection Tube | 10 | 50 |
Buffer CBS | 5 mL | 25 mL |
Solution Ⅰ | 12 mL | 60 mL |
Solution Ⅱ | 12 mL | 60 mL |
Solution Ⅳ | 10 mL | 50 mL |
Solution ePB | 12 mL | 60 mL |
Solution eW1 | 6 mL | 30 mL |
Wash Solution | 3 mL | 12 mL |
Elution Buffer | 5 mL | 15 mL |
RNase A (10 mg/mL) | 200 μL | 1 mL |
Protocol | 1 | 1 |
Storage
·RNase A: Store at -20 ℃. Other components are stable for 18 months at room temperature (15-25 ℃). For storage at elevated temperatures or extended shelf life, store at 2-8 ℃
Features
·Flexible and easy to operate.
·Yields 100-200 μg of high-concentration, endotoxin-free plasmid DNA. Recommended culture volumes: 10-20 mL LB medium or 6-10 mL 2YT medium.
