EndoFree Plasmid Midiprep Kit (Vacuum Filtration)
EndoFree Plasmid Midiprep Kit (Vacuum Filtration)
Product Composition
Components | GK2108 10 Preps | GK2108 40 Preps |
Genclean Column GP4 | 10 | 40 |
Collection Tube | 10 | 40 |
Extension tube | 10 | 40 |
Buffer CBS | 5 mL | 25 mL |
Solution Ⅰ | 33 mL | 130 mL |
Solution Ⅱ | 33 mL | 130 mL |
Solution Ⅳ | 27 mL | 105 mL |
Solution GETR | 13 mL | 50 mL |
Solution eDBS | 30 mL | 120 mL |
Wash Solution | 3 mL | 12 mL |
Enhanced Elution Buffer | 5 mL | 15 mL |
RNase A (10 mg/mL) | 500 μL | 2 mL |
Protocol | 1 | 1 |
Storage
·RNase A: Store at -20 ℃. Other components are stable for 18 months at room temperature (15-25 ℃). For storage at elevated temperatures or extended shelf life, store at 2-8 ℃
Features
·Negative-pressure filtration enables high-volume supernatant processing .
·Yields 200-300 μg of high-concentration, endotoxin-free plasmid DNA. Recommended culture volumes: 20-40 mL LB medium or 10-20 mL 2YT medium
