EndoFree Plasmid Maxiprep Kit (Spin Column)
EndoFree Plasmid Maxiprep Kit (Spin Column)
Product Composition
Components | GK2009 2 Preps | GK2009 10 Preps |
Genclean Column GP6 | 2 | 10 |
50-mL Collection Tube | 2 | 10 |
Push Filter | 2 | 10 |
Buffer CBS | 10 mL | 50 mL |
Solution Ⅰ | 25 mL | 120 mL |
Solution Ⅱ | 25 mL | 120 mL |
Solution Ⅳ | 20 mL | 100 mL |
Solution ePB | 25 mL | 120 mL |
Solution eW1 | 20 mL | 100 mL |
Wash Solution | 12 mL | 48 mL |
Elution Buffer | 10 mL | 30 mL |
Visual Blue | 200 μL | 600 μL |
RNase A (10 mg/mL) | 250 μL | 1200 μL |
Protocol | 1 | 1 |
Storage
·RNase A: Store at -20 ℃. Other components are stable for 18 months at room temperature (15-25 ℃). For storage at elevated temperatures or extended shelf life, store at 2-8 ℃
Features
·Simple and rapid operation.
·Small elution volume and high plasmid concentration.
·Endotoxin levels as low as 1 EU/μg to meet transfection requirements.
·Yields 1-4 mg of high-concentration, endotoxin-free plasmid DNA. Recommended culture volumes: 100-200 mL LB medium or 50-100 mL 2YT medium.
