Enhanced EndoFree Plasmid Maxiprep Kit (Vacuum Filtration)
Enhanced EndoFree Plasmid Maxiprep Kit (Vacuum Filtration)
Product Composition
Components | GK2110 2 Preps | GK2110 10 Preps |
Genclean Column GP7 | 2 | 10 |
50-mL Collection Tube | 2 | 10 |
Extension tube | 2 | 10 |
Buffer CBS | 10 mL | 50 mL |
Solution Ⅰ | 25 mL | 120 mL |
Solution Ⅱ | 25 mL | 120 mL |
Solution Ⅳ | 20 mL | 100 mL |
Solution GETR | 8 mL | 40 mL |
Solution eDBS | 40 mL | 200 mL |
Wash Solution | 12 mL | 48 mL |
Enhanced Elution Buffer | 10 mL | 30 mL |
Visual Blue | 200 μL | 600 μL |
RNase A (10 mg/mL) | 250 μL | 1200 μL |
Protocol | 1 | 1 |
Storage
·RNase A: Store at -20 ℃. Other components are stable for 18 months at room temperature (15-25 ℃). For storage at elevated temperatures or extended shelf life, store at 2-8 ℃
Features
·Deep endotoxin removal process for ultra-low endotoxin plasmids down to 0.05 EU/μg.
·Negative-pressure filtration enables more convenient operation.
·Yields 1-4 mg of high-concentration, endotoxin-free plasmid DNA. Recommended culture volumes: 100-200 mL LB medium or 50-100 mL 2YT medium.
