Enhanced EndoFree Plasmid Maxiprep Kit (Slim-Profile Spin Column)
Enhanced EndoFree Plasmid Maxiprep Kit (Slim-Profile Spin Column)
Product Composition
Components | GK2209 4 Preps | GK2209 20 Preps |
Genclean Column GP5 | 4 | 20 |
50-mL Collection Tube | 4 | 20 |
Push Filter (Optional) | 4 | 20 |
Buffer CBS | 10 mL | 50 mL |
Solution Ⅰ | 25 mL | 130 mL |
Solution Ⅱ | 25 mL | 130 mL |
Solution Ⅳ | 20 mL | 105 mL |
Solution GETR | 8 mL | 40 mL |
Solution eDBS | 40 mL | 240 mL |
Wash Solution | 12 mL | 48 mL |
Enhanced Elution Buffer | 10 mL | 35 mL |
Visual Blue | 200 μL | 600 μL |
RNase A (10 mg/mL) | 250 μL | 1200 μL |
Protocol | 1 | 1 |
Storage
·RNase A: Store at -20 ℃. Other components are stable for 18 months at room temperature (15-25 ℃). For storage at elevated temperatures or extended shelf life, store at 2-8 ℃
Features
·Small elution volume and high plasmid concentration.
·Deep endotoxin removal process for ultra-low endotoxin plasmids down to 0.05 EU/μg.
·Compared with conventional endoFree plasmid maxiprep kit, this kit reduces the number of centrifμgations by 2-4 times, which makes the operation faster.
·Yields 500-1500 μg of high-concentration, endotoxin-free plasmid DNA. Recommended culture volumes: 500-100 mL LB medium or 50 mL 2YT medium.
