Generay, one of the largest gene synthesis suppliers, has synthesized over 100,000 genes (500,000bp/month) for customers throughout the world. Rich experience, professional technology, powerful self-developed sequence analysis software and customer online tracking system allow us to consistently offer custom gene synthesis and related service at competitive price and with reliable delivery time. It is our mission to serve the customer with maximum satisfaction.

Advantages
●Professional team full of experience and creativity
Proprietary technology enable mutation-free synthesis for genes of any length and complexity

●Optimized gene expression level and improved protein solubility
Our proprietary Opti-gene software comprehensively optimizes factors critical to transcription, translation, and co-translational protein folding to deliver the highest possible levels of expression in any system.
●Any gene in any vector
Genneray's new recombination-based cloning system, can accurately and efficiently fuse your gene into any site on any vector in less than 30 minutes.
●Cost effective
Our competitive prices supported by our economy-of-scale service can ease your budget crunch.

Price

● Cloning into our standard vector pGH is free of charge.
● Extra $150.00 for subcloning into a commercial vector or a non-commercial vector customer supplied
● If for any reason we cannot subclone the gene into a non-standard vector, the gene will be delivered in pGH and all subcloning fee will be waived.
● Contact us for quotations for complex sequences.

Quotation and Ordering
● For quotation requests and questions, you may contact us by phone, email, fax, or via our online quotation system.
● Orders can be placed by phone, email, fax, or online with a formal PO (Purchase Order) or credit card.
● Our customer service representatives are available 24 hours Monday through Friday.

Contact:
E-mail: gene@generay.com.cn
　 　  sales@generay.com.cn

Tel: +86-21-67626120
Website: http://www.generay.com.cn/English

About complex gene
Low GC limitation:

● The lowest GC content should not be less than 20% for the full sequence. For a local region, which is about less than 100 bp, the GC content can be lower to 10%. For the very low GC region, there should not be contains other complex region, such as hairpin structure, reverse complementary repeat region. For this very low GC region, we generally divide into about 200 bp for synthesizing. This length is reliable for us to obtain right clone.

● Because very low GC sequence should be divided into small fragments for synthesizing, proper endonuclease restriction enzymes are needed. So in the sequence, BsaI ( ggtctc) and BpiI (gaagac) should be removed, usually these two enzyme are used as universal enzyme to divide the intact gene into small fragments. If there are other proper enzymes, it will be good.

● Because the full length must be divided into small fragments for synthesizing, and then it must be ligated together to get full length, the delivery time will be longer than normal sequence. Usually we will charge extra fees. In general, the price range depends on gene complexity, gene length and difficulty. （trouble shooting charge）

● Other special low GC sequence, please quote.

● In general, low GC content sequence will be reliable for synthesizing, because there is no problem for sequencing the final product, just higher cost and longer time.

● For GC content which is higher than 30%, in general, it is reliable for us to synthesize.


High GC limitation:
High GC sequence is different from low GC sequence, because it will be difficult to sequence final product. So 70% GC content for a full sequence, and 80% GC content for a partial region, such as less than 100 bp, are the limited. We usually do not accepted GC content higher than 75%, because usually we can not obtain sequencing result for them. For high GC content and especially long and high GC content sequence, please enquire separately. Also we need to divide high GC sequence into about 300 bp for synthesis, we should have proper endonuclease restriction enzymes, especially BsaI and BpiI restriction sites should be removed.


Reverse complementary repeat sequence
Generally, we do not accept the gene order with reverse complementary repeat sequence, because we can not sequence its final product. Reverse complementary repeat sequence is the most difficult structure for sequencing.