Molecular biology techniques are tiresome and time-consuming to most life science researchers. We have a wide range of ready-to-use molecular biology kits with consummate service system to help improve your efficiency.
Mutagenesis Service
Mutagenesis is central to molecular biology, facilitating research in a variety of fields, including studies of gene regulatory elements, DNA-protein interaction, protein structure/function, enzyme active sites, and novel proteins.
¡ñ Sequence Confirmation: The sequence of template DNA will be confirmed by sequencing upon request.
¡ñ Primer Design/Synthesis: Primer design and synthesis for mutagenesis are included.
¡ñ Mutation Introduction: Mutations are introduced by PCR.
¡ñ Mutant Screening: Every mutation is verified by sequencing.
¡ñ Mutant DNA Preparation: Generay can manufacture mutant DNA plasmid up to 1000 mg scale with required purity and endotoxin levels.
Sample Submission Requirements
Please provide following:
¡ñ The complete sequence of the template including the target gene and its vector
¡ñ Mutation specifications
¡ñ A sufficient amount of culture if the starting material is bacterial culture
¡ñ Maps and antibiotic resistance of the template and destination vectors
¡ñ A 2 ¦Ìg template DNA if the starting material is DNA
Delivery Specifications
Our typical turnaround is about 10 business days. Our delivery package includes:
¡ñ 5¦Ìg lyophilized destination vector containing the gene
¡ñ Sequence chromatograms covering your gene (electronic)
¡ñ Construct map for the plasmid (electronic)
¡ñ Quality assurance certificate
Price
Length of fragment |
Price ($) |
One mutation site |
Two mutation sites |
Three mutation sites |
<1000bp |
120.00 |
215.00 |
290.00 |
1000¡«3000bp |
150.00 |
260.00 |
350.00 |
>3000bp |
200.00 |
338.00 |
446.00 |
We will charge only one site if there are two mutation sites apart of 6bp; Please inquire if you have bulk sample or many of mutation sites.
Lead time
We will deliver in ten workdays from received plasmid, and contact with you timely if there are special conditions.
Cloning service
Sample Submission Requirements
PCR products, plasmid DNA, and bacterial culture are all accepted.
Please provide the following:
¡ñ The complete sequence of the template including the target gene and its vector
¡ñ If the starting material is DNA, a 2 ¦Ìg template DNA
¡ñ A sufficient amount of culture if starting material is bacterial culture
¡ñ Maps, antibiotic resistance sites, and cloning sites of destination vectors
Delivery Specifications
Our typical turnaround is about 14 business days depending on the complexity of the project. Our delivery package includes the following:
¡ñ 5¦Ìg lyophilized destination vector containing the gene
¡ñ Sequence chromatograms covering your gene (electronic)
¡ñ Construct map for the plasmid (electronic)
¡ñ Quality assurance certificate
TA Cloning
Clone the PCR products with 3¡¯ dA overhang to our pTG19-T vector, transformation, screening and sequencing.
Price
Length of fragment |
Price ($) |
Lead time |
<600 bp |
43.00 |
14 workdays |
600¡«1,500bp |
57.00 |
14 workdays |
1,500¡«3,000bp |
87.00 |
14 workdays |
>3000bp |
quotation |
Sub-cloning
Price
| Length of fragment |
Price ($) |
Lead time |
<1000bp |
143.00 |
14 workdays |
1000¡«2000bp |
286.00 |
14 workdays |
2000¡«3000bp |
quotation |
Quotation and Ordering
¡ñ For quotation requests and questions, you may contact us by email, phone, fax or via our online quotation system.
¡ñ Orders can be placed by phone, email, fax, or online with a formal PO (Purchase Order) or credit card.
¡ñ Our customer service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance.
Contact:
E-mail: gene@generay.com.cn
¡¡ ¡¡ ¡¡ order@generay.com.cn
Tel: +86-21-67626120
Web: http://www.generay.com.cn/English
¡¡¡¡
