|
 |
 |
| PCR Related Reagent > PCR Enzymes |
|
|
| Cat.No. |
Product information |
Quantity |
Price |
| GR0500 |
Taq DNA Polymerase |
200U/500U/1000U |
$9.0/ $20.0/ $37.0 |
| ET3010 |
Taq plus DNA Polymerase |
100U/200U/500U |
$9.0/ $16.0/ $36.0 |
| ET3020 |
Pfu DNA Polymerase |
100U/200U/500U |
$13.0/ $23.0/ $52.0 |
|
|
| Product Information: |
a. Taq DNA Polymerase
Description:
Taq DNA polymerase is a high thermostable DNA polymerase of a thermophilic bacterium Thermus aquaticus. The product is purified from E.coli cells carrying a cloned pol gene from Thermus aquaticus. It catalyzes 5'-3' synthesis of DNA. The enzyme has no detectable 3'-5' proofreading exonuclease activity, and possesses low 5'-3' exonuclease activity.
Components:
Taq DNA Polymerase, 5U/ul; 10xPCR Buffer; 20mM MgCl2 Solution.
Unit Definition:
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10mmols of dNTPs in to an acid-insoluble material in 30 min at 74℃.
Quality Control:
Tested for the absence of endonucleases, ribonucleases. SDS-PAGE purity passed.
Applications:
PCR amplification of DNA fragments as long as 6kb.DNA labeling. PCR for clone. |
|
b. Taq-plus DNA Polymerase
Description:
Taq-plus is a mixture of Taq and Pfu DNA Polymerase. Taq-plus is used to improve the reliability and yield of conventional primer extension reaction. It has two following advantages:High fidelity with an error frequency 1.6x10-6 during DNA synthesis.Taq plus increases the efficiency of polymerization reacion, resulting in a great percentage of extenuation reaction completion up to 10kb to 30kb. Pfu has a temperature optimum between 72-78℃ and remains more than 95% active following 1-hour incubation at 95℃.
Reaction Conditions:
All reagents, including Taq plus, should be mixed immediataly before use.
Typical reaction:
100ul of mixture containing 20-200uM dNTPs, 0.3-1uM primers, 0.1-0.25ng of template DNA, 10ul of 10x reaction buffer and 2.5-5 Units of Taq plus. |
|
c. pfu DNA Polymerase
Description:
Pfu DNA Polymerase is a thermostable enzyme of approximately 92kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75℃, catalyzing the polymerization of nucleotides into duplex DNA in the 5'-3' direction in the presence of magnesium. Pfu DNA Polymerase also possesses 3'-5' exonuclease (proofreading) activity. Base misinsertions that may occur during polymerization are rapidly excised by the proofreading activity of the polymerase. Consequently, Pfu DNA Polymerase is recommended for use in PCR and primer extension reactions that require high-fidelity synthesis. Pfu DNA Polymerase generated PCR fragments are blunt-ended.
Components:
Pfu DNA Polymerase, 5U/ul. 10xPCR Buffer with MgSO4.
Unit Definition: One unit of enzyme catalyzes the incorporation of 10nmol of deoxyribonucleotides into acid insoluble form at 75℃ for 30 minutes.
Quality Control:
SDS-PAGE purity assay passed. No endonuclease activity.
Applications:
PCR applications which demand high fidelity.High fidelity PCR for cloning into blunt-ended vectors. Site-directed mutagenesis.
Note:
The error rate of Pfu DNA Polymerase in PCR is 2.6x10-6 errors per nt per cycle. Do not use dUTP in PCR. |
|
 |
|
