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Construction of DNA Saturation
Mutagenesis Library (DNA library)
Controlled
randomization mutagenesis technique can be identified as a new technique in
protein engineering, by which the targeted amino acid can be replaced by
other 19 amino acids with random substitution. This technique is not only a
powerful tool for directed evolution of a certain protein with improved or
novel properties but also important method to research the relationship between
protein structure and function. We can process saturation mutagenesis in the
position associated with certain function of protein appointed by custom to
get a required DNA library.
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a brief demonstration of DNA library
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Applications
◇Improvement of protein activity
◇Improvement of industrial enzymes
◇Affinity maturation of antibodies
◇Prolonging plasma half-life of
therapeutics
◇Physicochemical improvement (e.g.
dissolubility, heat stabilization, substrate combination specialty)
◇Agricultural science, petrol chemistry,
bioengineer, gene expression and regulation
Advantages of GENERAY Biotech
◇Diversities of up to 1014 achievable
◇Highly directed and high specialty
◇Ultra fast delivery time (3 to 4 weeks)
◇Low ancillary mutation rate
◇Little silent mutation
Quality control
◇Bulk sequencing of amplified and cloned
library
◇Individual clone analysis (depends on
your requirement)
What we need
◇Sequence of amino acid with mutagenesis
position or gene sequence with template with expected mutagenesis position.
◇Name of clone vector and clone point.
◇Latin name of host cell.
What we deliver
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Item
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Product Specifications
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DNA library before clone
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5 μg DNA（with
5' and 3' restriction sites）in line
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DNA library after clone
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10 μg DNA (customer's vector) in plasmid,
Glycerol Stock
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The statistical analysis of the final library
includes:
◇Flanking sequence analysis
◇Even distribution of nucleotides at the
degenerated sites
◇RT-PCR to verify the number of distinct
molecules in the non-amplified library if necessary
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